- 產(chǎn)品描述
上海嶸崴達(dá)實(shí)業(yè)有限公司,專業(yè)經(jīng)營生命科學(xué)領(lǐng)域的科研試劑、儀器和實(shí)驗(yàn)室消耗品,
致力于向國內(nèi)生命科學(xué)研究人員提供*的產(chǎn)品和服務(wù)。
我們代理和經(jīng)銷的分子生物學(xué)試劑、生化試劑、免疫試劑、細(xì)胞培養(yǎng)試劑、制藥工業(yè)原料、實(shí)驗(yàn)儀器與消耗品、臨床診斷產(chǎn)品等,已被廣泛應(yīng)用于生命科學(xué)基礎(chǔ)與開 發(fā)研究、制藥、疾病診斷、人口與健康、生物技術(shù)等諸多領(lǐng)域。我們的客戶遍布大學(xué)、研究所、醫(yī)院、制藥公司、生物技術(shù)公司等單位。
主要經(jīng)營的試劑品牌:
Roche試劑,部分常用現(xiàn)貨,周三定貨,2-3周到貨。
GE試劑,耗材,部分常用現(xiàn)貨,周二,周五定貨,3周左右到貨。
R&D試劑,周三定貨,2-3周到貨。
SIGMA試劑,周一,五定貨,3周左右到貨。
Millipore試劑,每周三定貨,2-3周到貨。
Invitrogen試劑,部分現(xiàn)貨,周三定貨,2-3周到貨。
ABCAM試劑,周三定貨,2-3周到貨。
eBioscience試劑,部分常用現(xiàn)貨,周三定貨,2-3周到貨。
STANT CRUZ試劑,周三定貨,2-3周到貨。
Biolegend試劑,周三定貨,2-3周到貨。
Peprotech試劑,部分常用現(xiàn)貨,周三定貨,2-3周到貨。
USB試劑,常備現(xiàn)貨,周五定貨,4周到貨。
Amresco試劑,常備現(xiàn)貨,3-4周到貨。
羅氏原料,請來電來郵咨詢。
Thermo試劑,pierce, Hyclone部分常用現(xiàn)貨。
主要經(jīng)營的耗材品牌:
1. BIOHIT,單道移液半支消毒長期*,350/支。
2. BD Fa2. BD Falcon耗材,常用常備現(xiàn)貨。
3. BRAND,常備現(xiàn)貨
Protease from Streptomyces griseus ,P8811 - powder, BioReagent, suitable for mouse embryo cell culture, ≥3.5 units/mg solid
別名: Actinase E, Pronase E
CAS號: 9036-06-0
product line BioReagent form powder total impurities starch, essentially free suitability suitable for mouse embryo cell culture storage temp. −20°C
產(chǎn)品描述如下:
Application
Protease is typically used in nucleic acid isolation procedures in incubations of 0.5-3.0 hours supplemented with 0.2% sodium dodecyl sulfate and 10 mM EDTA.
Other Notes
An unusually non-specific protease.
Preparation Note
Collected from culture broth of S. griseus and purified by successive column procedures.
Quality
Contains approximay 25% calcium acetate.
Specificity
A mixture of at least three proteolytic activities including an extracellular serine protease. In general, serine proteases display a wide range of substrate specificities, which are believed to be mediated by an active site composed of one Asp, one His, and a Ser residue in the molecule. This enzyme prefers to hydrolyze peptide bonds on the carboxyl side of glutamic or aspartic acid.
Unit Definition
One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent).
Physical properties
Compley inactivated by heating above 80 °C for 15-20 minutes.